Journal: Molecules and Cells

Article Title: miR-101 Inhibiting Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma through Downregulating Girdin

doi: 10.14348/molcells.2016.2161

Figure Lengend Snippet: Overexpression of miR-101 inhibited the proliferation ability of HepG2 cells. HepG2 cells were transfected with miR-101 mimics or NC-mimics, and cells were divided into three groups: untreated HepG2 group, NC-HepG2 group and miR-101-HepG2 group. (A) Representative fluorescence microscopy picture (× 100, the two small upper pictures) and white light microscopy picture (× 100, the two small lower pictures) are shown in the lower left part. (B) RT-PCR method was used to detect the miR-101 expression levels in HepG2 group, the blank group and the negative control group after transfection of miR-101. The untreated group (HepG2 group) was set as 1. (C) CCK method was used to detect cell proliferation at specific time points, * P < 0.05, *** P < 0.001.

Article Snippet: Human hepatocellular carcinoma cell strain HepG2 (ATCC, USA) was purchased from Cell Biology Laboratory, Xiangya School of Medicine, Central South University.

Techniques: Over Expression, Transfection, Fluorescence, Microscopy, Light Microscopy, Reverse Transcription Polymerase Chain Reaction, Expressing, Negative Control

Journal: Molecules and Cells

Article Title: miR-101 Inhibiting Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma through Downregulating Girdin

doi: 10.14348/molcells.2016.2161

Figure Lengend Snippet: Overexpression of miR-101 inhibited HepG2 migration and invasion abilities. (A) The scratch test was used to detect cell migration rates at 0, 24, 48 h, and the representative microscopy picture (× 40) was shown in the upper left part. (B) The Transwell test method was used to detect cell invasion ability. The representative microscopy picture (× 100) was shown in the lower left part, *** P < 0.001.

Article Snippet: Human hepatocellular carcinoma cell strain HepG2 (ATCC, USA) was purchased from Cell Biology Laboratory, Xiangya School of Medicine, Central South University.

Techniques: Over Expression, Migration, Microscopy

Journal: Molecules and Cells

Article Title: miR-101 Inhibiting Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma through Downregulating Girdin

doi: 10.14348/molcells.2016.2161

Figure Lengend Snippet: Girdin is the target gene of miR-101. (A) Western blotting was used to detect the protein expression level of Girdin in HepG2 cells and β-actin was used as the internal control. (B) qRT-PCR was used to detect the expression levels of Girdin mRNA in different treatment groups. (C) Cells were divided into three groups: non-treated blank (HepG2), negative control (NC-HepG2), and hsa-mir-101 (miR-101-HepG2), and co-transfected with either the WT Girdin reporter vector (Girdin) or the mutant (Girdin-Mut) vector. Fluorescence activities in different groups were detected. The normalized luciferase activity in the control group was set to 1. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Human hepatocellular carcinoma cell strain HepG2 (ATCC, USA) was purchased from Cell Biology Laboratory, Xiangya School of Medicine, Central South University.

Techniques: Western Blot, Expressing, Control, Quantitative RT-PCR, Negative Control, Transfection, Plasmid Preparation, Mutagenesis, Fluorescence, Luciferase, Activity Assay

Journal: Molecules and Cells

Article Title: miR-101 Inhibiting Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma through Downregulating Girdin

doi: 10.14348/molcells.2016.2161

Figure Lengend Snippet: Silencing Girdin expression inhibited proliferation ability of HepG2 cells. (A) The plasmid structure of Talen vector. (B) HepG2 cells were transfected plasmids in order to knockout of the Girdin gene. Forty-eight hours later Girdin expression levels were determined by western blotting. (C) CCK method was used to detect proliferation of HepG2 cell at indicated time points. * P < 0.05, *** P < 0.001 (NC vs. Girdin-KO), ## P < 0.01, ### P < 0.001 (Girdin-KO+miR-101 inhibitor vs. Girdin-KO).

Article Snippet: Human hepatocellular carcinoma cell strain HepG2 (ATCC, USA) was purchased from Cell Biology Laboratory, Xiangya School of Medicine, Central South University.

Techniques: Expressing, Plasmid Preparation, Transfection, Knock-Out, Western Blot

Journal: Molecules and Cells

Article Title: miR-101 Inhibiting Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma through Downregulating Girdin

doi: 10.14348/molcells.2016.2161

Figure Lengend Snippet: Silencing Girdin expression inhibited migration and invasion abilities of HepG2 cells. (A, B) The scratch test was used to measure migration ability of HepG2 cell in different treatment groups. (C, D) The Transwell test was performed to detect cells invasion ability in different treatment groups. KO: knockout, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Human hepatocellular carcinoma cell strain HepG2 (ATCC, USA) was purchased from Cell Biology Laboratory, Xiangya School of Medicine, Central South University.

Techniques: Expressing, Migration, Knock-Out